HPLC
, comparison of stationary phases, C
18
-phases vs. polystyrene-phases (
IH
Development and investigation of new HPLC-, microbore- and capillary HPLC-colums (
EG
).
Gas chromatographie
analysis (incl. capillary column preparation - in particular with optically active stationary phases); gas chromatographic/massspectrometric analysis.
Amino acid analysis (gas chromatography): quant. analysis by enantiomer labelling; determination of optical purity of amino acids, aa. derivatives and peptides incl. elimination of hydrolysis-induced racemisation by deuterium exchange/ GC/MS detection; structural elucidation of peptidic materials by GC and GC/MS (
GN
).
Analytic of antisense oligonucleotides by multiple
CE-methodes
peptides and proteines; Drug analysis in cooperation with Dr. Schneeberger (Bürgerhospital/Stuttgart) and the group of Prof. Dr. Kovar (Tübingen);
CE-ES-MS
and
CE-NMR
coupling (
JS
).
Analytic of
hop bitter acids
by
HPLC, NMR
and
LC-NMR
and LC-MS coupling;
quantitative analysis by NMR and HPLC-NMR coupling; structural elucidation of biomolecules and natural products by NMR; CHPLC-, CE and CEC-NMR-coupling in cooperation with Prof. K. Albert (Tuebingen) (
KP
).
Peptide, protein, oligonucleotide and chiral separation by
electrochromatography
(CEC),
capillary HPLC
and their coupling with
ES-MS
and
NMR
(
PG
).
Sequencing
of biomolecules (peptides, proteins, oligodeoxynucleotides, oligophosphor-thioates, oligophosphordithioates) by
mass spectrometry
;
Coupling of ES-MS
with chromatographic methods
(capillary HPLC, capillary electrophoresis electro-chromatographie); Studies of
non-covalent complexes (
enzyme-substrate, peptide-nucleotide interactions, oligonucleotide duplexes, peptide-metal complexes, cyclodextrine complexes);
Drug metabolism
studies
(
RL; CR
).
References
B. Behnke, G. Schlotterbeck, U. Tallarek, S. Strohschein, L.-H. Tseng, T. Keller, K. Albert, E. Bayer,
Anal. Chem.
A. Höltzel, G. Schlotterbeck, K. Albert, E. Bayer, Seperation and Characterisation of Hop Bitter Acids by HPLC-
1
H NMR Coupling,
Chromtographia
K. Schmeer, B. Behnke, E. Bayer,
Anal. Chem.
B. Behnke, E. Bayer,
J. Chromatogr. A
G. Piperopoulos, R. Lotz, A. Wixforth, T. Schmierer, K.-P. Zeller, Determination of naphthodianthrones in plant extracts from
Hypericum perforatum L.
by liquid chromatography-electrospray ionization mass spectrometry,
J. Chromatogr. B
M. Dahms, R. Lotz, W. Lang, U. Renner, E. Bayer, H. Spahn-Langguth, Elucidation of phase-I-and phase-II-metabolic pathways of rhein: species-differences and their potential relevance in toxicology,
J. Drug. Metab. Disp
H. Drechsel, H. Stephan, R. Lotz, H. Haag, H. Zähner, K. Hantke, G. Jung, Structure elucidation of Yersiniabactin, a siderophore from highly virulent
Yersinia
strains,
Liebigs Ann
., 1727-1733, 1995
Index
Conversion
Oligonucleotides
Peptides
Polymeres
Sidelines